混頻交流磁導率應用於生物分子C反應蛋白之檢測

Abstract

C反應蛋白(C-Reactive Protein，CRP)是一種在發炎時期由肝臟生成的血漿蛋白，研究指出C反應蛋白會在有感染、發炎或損傷時，在血漿含量較高。 本研究利用具有超順磁特性的奈米磁性粒子，並將具有高靈敏度與穩定度的C反應蛋白披覆於奈米磁性粒子上，利用抗原與抗體互相結合之特性，進行交流混頻磁導率免疫檢測。 原線圈架構只能透過一個線圈調整梯度線圈的磁場平衡度，量測原線圈架構的訊雜比為4.26，且在雜訊部分，原線圈架構的數值為158 µV，已經影響到了樣品訊號。為了改善此狀況，所以本實驗重新設計了線圈架構，將可調整的線圈數增加為兩個，以提高梯度線圈的磁場平衡度，進一步降低背景訊號對擷取訊號的影響，使得到的量測訊號，可以趨近於樣品訊號，新線圈架構所量測到的訊雜比為16.89，且在雜訊部分，新線圈架構的數值下降至0.05 µV，改善了原線圈架構發生的問題。C-reactive protein (CRP) is an acute phase reactant which is produced by liver during the episode of acute inflammation, whose levels rise in response to inflammation. In this study, we used magnetic nanoparticles with superparamagnetic properties. C-reactive protein with high sensitivity and stability coat on magnetic nanoparticles. We use the characteristics of the combination of antigen and antibody to perform Mixing frequency Alternating Current Magnetic Susceptibility. The original coil structure uses a coil to adjust the magnetic field balance of the gradient coil, and the signal-to-noise ratio of the original coil structure is 4.26. The noise part, with a value of 158 μV, has affected the sample signal. In order to better this situation, this experiment redesigned the coil structure and increased the number of adjustable coils to two to increase the magnetic field balance of the gradient coil, further reducing the effect of the background signal on the pick-up signal, So that the measured signal can be approached to the sample signal. The new coil structure has a signal-to-noise ratio of 16.89. In the noise part, the value drops to 0.05 μV, which improves the problem with the original coil structure.