以大腸桿菌生合成之對胺基苯甲酸定量水中硝酸鹽及亞硝酸鹽

dc.contributor葉怡均zh_TW
dc.contributorYeh, Yi-Chunen_US
dc.contributor.author張嘉雯zh_TW
dc.contributor.authorChang, Chia-Wenen_US
dc.date.accessioned2023-12-08T07:54:21Z
dc.date.available2023-07-31
dc.date.available2023-12-08T07:54:21Z
dc.date.issued2023
dc.description.abstract我們實驗室提出以全細胞生物感測器來有效檢測並定量水中的硝酸鹽及亞硝酸鹽的方法,參考葡萄糖在大腸桿菌中的代謝路徑建構之質體轉殖入 E. coli BL21(DE3),其製造的T7 RNA polymerse (RNAP)會開啟T7啟動子製造下游蛋白,這些蛋白會加速葡萄糖代謝成為4-Aminobenzoic acid (PABA),使我們在短時間內就可以得到大量PABA,其經由一系列反應得到最終產物在540 nm有最大吸收的色素,以吸收回推濃度、再以大腸桿菌還原硝酸鹽為亞硝酸鹽後並以同樣反應定量出硝酸鹽濃度。zh_TW
dc.description.abstractIn this study, we propose a method for the efficient detection and quantification of nitrate and nitrite in water using biosynthesis product 4-Aminobenzoic acid (PABA). Referring to the metabolic pathway of glucose in E. coli, the plasmid constructed is transformed into E.coli BL21(DE3). The T7 RNA polymerse (RNAP) produced by E. coli BL21(DE3) will turn on the T7 promoter to produce downstream proteins, which will accelerate glucose metabolism into PABA, so that we can obtain a large amount of PABA in a short time. PABA passes through a series of reactions to obtained the pigment as final product, which the maximum absorbance is at 540 nm. The concentration is calculated by the absorbance, and the nitrate is reduced by E. coli and quantified by the same reaction.en_US
dc.description.sponsorship化學系zh_TW
dc.identifier61042090S-43703
dc.identifier.urihttps://etds.lib.ntnu.edu.tw/thesis/detail/de9ed2b25f41fd5999e90788f0f5f0ce/
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/121020
dc.language中文
dc.subject硝酸鹽zh_TW
dc.subject亞硝酸鹽zh_TW
dc.subject定量檢測zh_TW
dc.subject比色法zh_TW
dc.subjectnitrateen_US
dc.subjectnitriteen_US
dc.subjectquantitative detectionen_US
dc.subjectcolorimetryen_US
dc.title以大腸桿菌生合成之對胺基苯甲酸定量水中硝酸鹽及亞硝酸鹽zh_TW
dc.titleDetection and Quantification of Nitrate and Nitrite in Water Using PABA Produced by Escherichia colien_US
dc.typeetd

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